中成药

目的 建立超高效液相色谱-三重四极杆/复合线性离子阱质谱(UHPLC-QTRAP-MS)法同时测定助阳护心膏中毛蕊异黄酮葡萄糖苷、芒柄花素、芒柄花苷、毛蕊异黄酮、莪术二酮、次乌头碱、苯甲酰次乌头原碱、芍药内酯苷、槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷、红景天苷、山柰酚、芍药苷的含量。方法 分析采用Poroshell EC-C₁₈色谱柱(100 mm×2.1 mm, 1.9μm);流动相乙腈(含0.1%甲酸)-水(含0.1%甲酸),梯度洗脱；体积流量0.3 mL/min;柱温35℃;电喷雾离子源；正负离子扫描；多反应监测模式。结果 12种成分在各自范围内线性关系良好(r≥0.995 0),平均加样回收率94.60%～109.47%,RSD 2.26%～4.95%。结论 该方法简便、准确、可靠，可用于助阳护心膏的质量控制。

AIM To establish a ultra-performance liquid chromatography-triple quadrupole/composite linear ion trap mass spectrometry(UHPLC-QTRAP-MS) method for the simultaneous content determination of calycosin-7-glucoside, formononetin, ononin, calycosin, curdione, hypaconitine, benzoylhypacoitine, albiflorin, quercetin 3-O-β-D-glucose-7-O-β-D-gentiobioside, salidroside, kaempferol and paeoniflorin in Zhuyang Huxin Ointment.METHODS The analysis was performed on a 35 ℃ thermostatic Poroshell EC-C₁₈ column(100 mm×2.1 mm, 1.9 μm), with the mobile phase comprising of acetonitrile(containing 0.1% formic acid)-water(containing 0.1% formic acid) flowing at 0.3 mL/min in a gradient elution manner, and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Twelve constituents showed good linear relationships within their own ranges(r≥0.995 0), whose average recoveries were 94.60%-109.47% with the RSDs of 2.26%-4.95%.CONCLUSION This simple, accurate and reliable can be used for the quality control of Zhuyang Huxin Ointment.