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目的 建立UPLC-Q-TOF-MS/MS法鉴定没食子酸、鞣花酸、柯里拉京在小鼠体内的代谢产物。方法 给药组小鼠分别灌胃给予3种成分的0.5%CMC-Na混悬液(400 mg/kg),空白组小鼠灌胃给予0.5%CMC-Na混悬液(400 mg/kg),采集血浆、尿液、粪便。分析采用Acquity UPLC HSS T3色谱柱(100 mm×2.1 mm, 1.8μm);流动相水(含0.1%甲酸)-乙腈，梯度洗脱；体积流量0.27 mL/min;柱温30℃;电喷雾离子源；负离子扫描。结果 共鉴定出27种没食子酸代谢产物、13种鞣花酸代谢产物、37种柯里拉京代谢产物，这3种成分主要发生水解、脱羧等Ⅰ相代谢，以及甲基化、硫酸化、葡萄糖醛酸化等Ⅱ相代谢。结论 该方法稳定可靠，可为探究其他天然酚类的体内代谢规律提供参考。
Abstract：AIM To establish a UPLC-Q-TOF-MS/MS method for the identification of in vivo metabolites of gallic acid, ellagic acid and corilagin in mice.METHODS The mice in administration groups were given intragastric administration of 0.5% CMC-Na suspensions of 3 constituents(400 mg/kg), respectively, while those in blank group were given intragastric administration of 0.5% CMC-Na suspensions(400 mg/kg), after which plasma, urine and feces were collected. The analysis was performed on a 30 ℃ thermostatic Acquity UPLC HSS T3 column(100 mm×2.1 mm, 1.8 μm),with the mobile phase comprising of water(containing 0.1% formic acid)-acetonitrile flowing at 0.27 mL/min in a gradient elution manner, and electrospray ionization source was adopted in negative ion scanning.RESULTS Total 27 gallic acid metabolites, 13 ellagic acid metabolites and 37 corilagin metabolites were identified, these 3 constituents mainly occured phase Ⅰ metabolites such as hydrolysis, decarboxylation, and phase Ⅱ metabolites such as methylation, sulfation, glucuronidation.CONCLUSIONThis stable and reliabe method can provide a reference for exploring the in vivo metabolic rules of other natural phenols.
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基本信息:
中图分类号:R285.5
引用信息:
[1]赵蓉,胡蒙蒙,洪玉冰,等.UPLC-Q-TOF-MS/MS法鉴定3种酚类在小鼠体内的代谢产物[J].中成药,2026,48(01):33-41.
基金信息:
兴滇英才支持计划项目(20220273); 云南省基础研究计划项目面上项目(202401AT070160); 云南省科技厅-云南中医药大学应用基础联合研究专项项目面上项目(202101AZ070001-212);云南省科技厅-云南中医药大学应用基础联合研究专项项目重点项目(2019FF002-012)
2026-01-20
2026-01-20